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RNA-Seq Expression Analysis from De Novo Transcriptome Assembly Projects

The growing availability and reduced cost of next-generation sequencing (NGS) has made RNA-Seq an appealing tool for analyzing the expression of genes, alternatively spliced transcripts and other genomic elements. However, the vast quantity and complexity of the NGS data is preventing researchers from taking full advantage of this powerful technology for RNA-Seq expression analysis. Furthermore, while a large number of genomes are available in public repositories, a greater number of species do not yet have a reference genome suitable for RNA-Seq expression analysis. In this case, a relatively low-cost solution is to generate an annotated transcriptome and use it as a reference for down-stream expression analysis.

In this Case Study, we share with you how we can provide researchers with the tools necessary to plan and execute an RNA-Seq expression analysis from de novo transcriptome assembly projects.

RSEM Expression Calculation

For the complete results of the analysis, please download the white paper here.